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ilvB [2017-8-11 16:0:3]
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ilvB [2017-8-11 16:0:3]

acetolactate synthase (large subunit)
Locus
BSU28310
Isoelectric point
5.18
Molecular weight
62.42 kDa
Protein length
574 aa Sequence Blast
Gene length
1722 bp Sequence Blast
Function
biosynthesis of branched-chain amino acids
Product
acetolactate synthase (large subunit)
Essential
no
E.C.
2.2.1.6
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,895,248 → 2,896,972

The protein

Catalyzed reaction/ biological activity

  • 2 pyruvate = 2-acetolactate CO2 (according to Swiss-Prot)
  • Protein family

  • TPP enzyme family (according to Swiss-Prot)
  • Paralogous protein(s)

    Modification

  • phosphorylated on Arg-297 PubMed
  • Structure

  • 4RJJ PubMed
  • 1N53 (T box RNA)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • T-box: termination/antitermination, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon
  • Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed
  • Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • GP324, ilvB under control of pXyl (cat), available in Jörg Stülke's lab
  • LacZ fusion

  • pGP520 and pGP524 (both in pAC5), pGP523 (in pAC6), all are available in Jörg Stülke's lab
  • References

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